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KMID : 0613819970070020112
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Journal of Life Science
1997 Volume.7 No. 2 p.112 ~ p.118
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Bioconversion of Pytogallo from Gallic Acid by Pseudomonas sp. KS-96 using Rotating Disc Contactor
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Abstract
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In previous paper Pseudomonas sp. Ks-96 isolated from the bioconversion into pyrogallol from gallic acid . Continuous bioconversion of pyrogallol was carried out using rotatory disc contactor immobilized Pseudomonas sp. Ks-96 . Enzyme activity of gallate decarboxylase released from Pseudomonas sp. Ks-96 were shown at the highest activity on 24h incubation. Culture media containing gallic acid supplied on the flow rate of 20ml/h until thickness of cells wall reached steady state. Bioconversion rate of pyrogallol from gallic acid showed at highest level ranging from 18hr to 36h according to time courses. Continuous bioconversion of pyrogallol using rotating disc contactor was about 82% and 80% between 6 and 8 days at the feeding rate of 300ml per hour in the medium containing 15g/l gallic acid.
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